Western Blotting HRP Substrate

Lumigen ECL Plus (PS-3) is an acridan-based technology chemiluminescent and chemifluorescent substrate for sensitive detection of Western blotted proteins that are bound to horseradish peroxidase (HRP) labeled antibodies.

• Highly sensitive - approximately 5 pg per band of target protein on Western blot
• Sustained luminescence - signal is sustained for over several hours
• Dual signal functionality - chemiluminescent and chemifluorescent signals are generated simultaneously allowing detection on x-ray film or with a digital imager

Lumigen ECL Plus Technology

Lumigen ECL Plus utilizes a unique acridan based technology for the chemiluminescent and fluorescent detection of horseradish peroxidase (HRP) conjugates. The reaction involves the enzymatic generation of thousands of acridinium ester intermediates per minute which react with peroxide at alkaline pH to produce sustained high-intensity chemiluminescence. Additionally, the reaction end-product exhibits a long-lasting fluorescent signal which can be detected on a CCD imaging device.