Glucagon ELISA

(10-1271-01)

Kit format: 1×96 wells

Measurement range: 1.5-130 pmol/L (5-453 pg/mL)

1.No purification of samples

2.No cross-reactivity to glicentin, proglucagon 1-16, and glucagon 3-29

3.Highly appreciated and often cited assay

4.CE/IVD labeled on select markets

5.Controls available: 10-1286-01

DESCRIPTION

The Mercodia Glucagon ELISA is a CE/IVD-labelled, highly specific and sensitive immunoassay for measuring glucagon in plasma and serum.

The highly specific monoclonal antibodies used in this assay enable accurate determination of glucagon without significant cross-reactivity to other circulating pro-glucagon-derived peptides, e.g. glicentin.

As of October 1st, 2020, the assay protocol of Mercodia Glucagon ELISA 10-1271-01 is updated to make the sequential protocol default, abolishing cross-reactivity to glicentin. To run the old, simultaneous protocol, please refer to Technical Note TN34-161.

TEST CHARACTERISTICS

SPECIES：Human

SAMPLE TYPES：Serum, EDTA plasma and cell culture media

SAMPLE VOLUME：25 µL

ASSAY RANGE：1.5-130 pmol/L (5.95-453pg/mL)

DETECTION LIMIT：≤0.75 pmol/L

INCUBATION：18-22 h (overnight) + 1 h + 30 min

DETECTION METHOD：Absorbance

SPECIFICITY

The following cross-reactions have been tested:

GLICENTIN：Not detected

OXYNTOMODULIN：4.0 %

PROGLUCAGON (1-61)：Not detected

GLUCAGON (3-29)：Not detected